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The clinical isolate Staphylococcus aureus SA2 had four kinds of plasmids and was resistant to ampicillin, chloroamphenicol, clindamycin. erythromycin, gentamicin, kanamycin, methicillin, streptomycin, tetracycline and tobramycin. Transformation experiment demonstrated that 4.14kb plasmid(pKH7) encoded resistance to chloramphenicol. The cleavage map of pKH7 was determined by restriction enzyme mapping techniques. The cleavage map is given for BstEII, HindIII, HpaII, and XbaI. The above restriction endonucleases have a single site, but nucleases BamHI, BglI, BglII, EcoRI, EcoRV, HaeIII, HpaI, KpnI, PstI, PvnIl, SalI, SmaI, and XhoI have no site on this plasmid.
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